- DOS(Disk Operating System)
- DOS/VS(Disk Operating System / Virtual Storage)
- DOS/VSE(Disk Operating System / Virtual Storage Extended)
- DOT(Department of Transportation)
- DOTA(short-form for DEFENSE OF THE ANCIENTS a popular online epic game)
- DOTC(Dakota Ojibway Tribal Council)
- DotD(Deal of the Day (marketing gimmick))
- DoTE(Department of Technical Education)
- DOTEO(Department of Transportation Emergency Organization)
- DOTLMS(Doctrine, Organizations, Training, Leader Development, Materiel, and Soldiers)
- DOTMLFP(doctrine, organization, training, maintenance, leadership, personnel, and facilities)
- DOTMLPF(Doctrine, Organization, Training, Materiel, Leadership, Personnel, and Facilities (mnemonic))
- DOTS(Digital-Optical Technology System)
- DOTSP(Doctrinal and Organizational Test Support Package)
- DOTT(The Domestication Of Thunnus Thynnus)
- DOU(Dourados Airport)
- Double Dip(Term used when in a dual status and receiving income from two sources (e.g. Military Technician on civilian leave and orders))
- DOV(Dover Air Force Base)
- DOVE(Defenders of the Ouse Valley and Estuary)
